Human IL1R1 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL1R1 in cell culture supernates and serum.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL1R1
Protein name: Interleukin-1 receptor type 1
Gene Full Name: interleukin 1 receptor, type I
abbreviation: IL1R1
Background: Interleukin 1 receptor, type I(IL1R1), also known as CD121a(Cluster of Differentiation 121a), is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. IL1R1 along with interleukin 1 receptor, type II(IL1R2), interleukin 1 receptor-like 2(IL1RL2), and interleukin 1 receptor-like 1(IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. This protein is a receptor for interleukin 1 alpha(IL1A), interleukin 1 beta(IL1B), and interleukin 1 receptor antagonist(IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses.
ID:P14778
ID:3554

alias

IL-1R-1|IL-1RT-1|IL-1RT1|CD121 antigen-like family member A|Interleukin-1 receptor alpha|IL-1R-alpha|Interleukin-1 receptor type I|p80|CD_antigen: CD121a|L1R|IL1RA|IL1RT1|CD121A|D2S1473

Origin

Nativ and recombinant human IL1R1

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, D21-T332

sensitivity

<10pg/ml

detection range

125pg/ml-8000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|746|3374|5587|857|3345|6558
Standard deviation|30.59|175.5|312.9|59.13|250.9|518.1
CV(%)|4.1|5.2|5.6|6.9|7.5|7.9

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL1R1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1R1 has been precoated onto 96-well plates. Standards(NSO, D21-T332) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1R1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1R1 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human IL1R1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IL1R1 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|125|250|500|1000|2000|4000|8000 O.D.|0.088|0.123|0.199|0.307|0.484|0.829|1.454|2.511

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL1R1 antibody|1
ST0000-10|Lyophilized recombinant human IL1R1 standard|10ng/tubex2
AR1107|Biotinylated anti- human IL1R1 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4