For the quantitative determination of Human IL10/IL-10/Interleukin-10 concentration in cell culture supernates.
Human peripheral blood mononuclear cells (2 x 10E6 cells/mL) were cultured in 1640 supplemented with 10% bovine calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate, stimulated with 1 µg CD3(OKT3) for 3days. Aliquots of the cell culture supernates were removed and assayed for levels of natural human IL10, and measured 1.29 ng/mL.
This assay recognizes both recombinant and natural Human IL10. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL10Antigene: 10947-HNAEcapture antibody type: mouse mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||23.44-1500 pg/ml|