Human IL-6R alpha PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-6R alpha in cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL-6R alpha
Protein name: Interleukin-6 receptor subunit alpha
Gene Full Name: interleukin 6 receptor
abbreviation: IL6R
Background: IL6R alpha(IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit(IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.
ID:P08887
ID:3570

alias

CD126|CD126 antigen|Gp80|IL 6R 1|IL 6R alpha|IL-6 receptor subunit alpha|IL-6R 1|IL-6R subunit alpha|IL-6R-1|IL-6R-alpha|IL-6RA|Il6r|IL6R1|IL6RA|IL6RA_HUMAN|IL6Ralpha|Interleukin 6 receptor|Interleukin 6 receptor alpha chain|Interleukin-6 receptor subunit alpha|Membrane glycoprotein 80|MGC104991|OTTHUMP00000034320

Origin

Nativ and recombinant human IL-6R alpha

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

sf21, L20-D358

sensitivity

<10pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|115|768|1558|124|845|1334
Standard deviation|9.2|26.11|60.76|10.54|47.32|81.37
CV(%)|8|3.4|3.9|8.5|5.6|6.1

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-6R alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6R alpha has been precoated onto 96-well plates. Standards(sf21, L20-D358) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6R alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-6R alpha amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human IL-6R alpha standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IL-6R alpha standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.031|0.094|0.157|0.315|0.587|1.032|1.701|2.469

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-6R alpha antibody|1
ST0000-10|Lyophilized recombinant human IL-6R alpha standard|10ng/tube�2
AR1107|Biotinylated anti- human IL-6R alpha antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4