Human IL-6 sR ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human IL-6 sR ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-6 sR in Human serum, plasma and cell culture media. An antibody specific for Human IL-6 sR was coated on a 96-well plate. Standards and samples are added to the wells and any IL-6 sR present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human IL-6 sR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-6 sR present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cancer Biomarker; inflammation;

specific against

Human

detection target

interleukin 6 receptor(IL-6 sR)
Background: IL-6 R alpha is a transmembrane subunit of the receptor for Interleukin-6. It associates with gp130 which is a shared component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. IL-6 plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer.In melanocytes IL6R gene expression may be regulated by MITF.

alias

CD126, IL-6R-1, IL-6RA, IL6QA, IL6RQ, gp80, IL6R

sensitivity

2 pg/ml

detection range

4.1-1000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P08887