Human IL-6 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-6 in cell culture supernates, serum and plasma( heparin, EDTA, citrate).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL-6
Protein name: Interleukin-6
Gene Full Name: interleukin 6
abbreviation: IL6
Background: The human interferon-beta 2 gene(IFNB2) product is identical to that for the B-cell stimulation factor-2(BSF-2), the hybridoma growth factor(HGF)("interleukin-6"), and the hepatocyte stimulating factor(HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury(acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6(IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.
ID:P05231
ID:3569

alias

B cell differentiation factor| B cell stimulatory factor 2| B-cell stimulatory factor 2| BSF 2| BSF-2| BSF2| CDF| CTL differentiation factor| Cytotoxic T cell differentiation factor| Hepatocyte stimulating fact| HGF| HPGF| HSF| Hybridoma growth factor| Hybridoma plasmacytoma growth factor| IFN-beta-2| IFNB2| IL 6| IL-6| IL6| IL6 protein| IL6_HUMAN| Interferon beta 2| Interferon beta-2| Interleukin 6| Interleukin 6 (interferon beta 2)| Interleukin BSF 2| Interleukin-6| Interleukin6

Origin

Nativ and recombinant human IL-6

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, P29-M212

sensitivity

<0.3pg/ml

detection range

4.69pg/ml-300pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|16.3|98|179|18.2|99|185
Standard deviation|0.8|2.3|4.2|1.0|3.6|5.7
CV(%)|4.9|2.3|2.3|5.5|3.6|3.1

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6 has been precoated onto 96-well plates. Standards(E.coli, P29-M212) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-6 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 300pg/ml, 150pg/ml, 75pg/ml, 37.5pg/ml, 18.75pg/ml, 9.38pg/ml, 4.69pg/ml human IL-6 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each human IL-6 standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|4.69|9.38|18.75|37.5|75|150|300 O.D.|0.002|0.059|0.106|0.226|0.428|0.736|1.372|2.279

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-6 antibody|1
ST0000-10|Lyophilized recombinant human IL-6 standard|10ng/tubex2
AR1107|Biotinylated anti- human IL-6 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4