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Boster
For quantitative detection of human IL-5 in cell culture supernates, serum, plasma (heparin, EDTA) and urine.
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL-5Protein name: Interleukin-5Gene Full Name: interleukin 5abbreviation: IL5Background: Interleukin-5(IL-5) is also known as eosinophil differentiation factor(EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology. ID:P05113 ID:3567
B cell differentiation factor I| B-cell differentiation factor I| Colony stimulating factor eosinophil| EDF| Eosinophil differentiation factor| IL 5| IL-5| IL5| IL5_HUMAN| Interleukin 5| Interleukin 5 (colony stimulating factor eosinophil)| Interleukin-5| Interleukin5| T cell replacing factor| T-cell replacing factor| TRF
Nativ and recombinant human IL-5
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
sf21, I20-S134
<2pg/ml
7.8pg/ml-500pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|44.6|168|338|43.8|188|329 Standard deviation|2.32|6.89|14.87|2.63|10.34|21.06 CV(%)|5.2|4.1|4.4|6|5.5|6.4
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-5 has been precoated onto 96-well plates. Standards(sf21, I20-S134) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-5 amount of sample captured in plate.
Aliquot 0.1ml per well of the 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml human IL-5 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human IL-5 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|7.8|15.6|31.3|62.5|125|250|500 O.D.|0.012|0.065|0.113|0.223|0.440|0.814|1.439|2.332
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-5 antibody|1ST0000-10|Lyophilized recombinant human IL-5 standard|10ng/tubex2AR1107|Biotinylated anti- human IL-5 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <2pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-5 (IL5) |
Detection Range | 7.8pg/ml-500pg/ml |
Alias | B cell differentiation factor I, B-cell differentiation factor I, Colony stimulating factor eosinophil, EDF, Eosinophil differentiation factor, IL 5, IL-5, IL5, IL5_HUMAN, Interleukin 5, Interleukin 5 (colony stimulating factor eosinophil), I |