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Boster
For quantitative detection of human IL-33 in cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates.
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL-33Protein name: Interleukin-33Gene Full Name: interleukin 33abbreviation: IL33Background: Interleukin 33(IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell(CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2. ID:O95760 ID:90865
C9orf26| DVS27| DVS27 related protein| IL-1F11| IL-33| IL1F11| IL33| IL33_HUMAN| Interleukin-1 family member 11| Interleukin-33| NF-HEV| NFEHEV| Nuclear factor from high endothelial venules| RP11-575C20.2
Nativ and recombinant human IL-33
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, S112-T270
<10pg/ml
15.6pg/ml-1000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|74|383|644|91|411|738 Standard deviation|3.48|19.15|39.3|5.2|22.61|49.45 CV(%)|4.7|5|6.1|5.7|5.5|6.7
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-33 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-33 has been precoated onto 96-well plates. Standards(E.coli, S112-T270) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-33 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-33 amount of sample captured in plate.
Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human IL-33 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or cell lysates to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human IL-33 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.029|0.057|0.101|0.189|0.335|0.632|1.152|2.098
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-33 antibody|1ST0000-10|Lyophilized recombinant human IL-33 standard|10ng/tubex2AR1107|Biotinylated anti- human IL-33 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-33 (IL33) |
Detection Range | 15.6pg/ml-1000pg/ml |
Alias | C9orf26, DVS27, DVS27 related protein, IL-1F11, IL-33, IL1F11, IL33, IL33_HUMAN, Interleukin-1 family member 11, Interleukin-33, NF-HEV, NFEHEV, Nuclear factor from high endothelial venules, RP11-575C20.2 |