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Boster
For quantitative detection of human IL-29 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL-29Protein name: Interferon lambda-1Gene Full Name: interferon, lambda 1abbreviation: IFNL1Background: Interferon lambda-1, also called Interleukin-29(IL-29) is a protein that in humans is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. ID:Q8IU54 ID:282618
Cytokine ZCYTO21| IFN lambda 1| IFN-lambda-1| IFNL1| IL 29| IL-29| IL29| IL29_HUMAN| Interferon lambda 1| Interferon lambda-1| Interferon, lambda 1| Interleukin 29| Interleukin-29
Nativ and recombinant human IL-29
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
NSO, G20-T200
<10pg/ml
62.5pg/ml-4000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|517|1446|2336|643|1550|2468 Standard deviation|33.1|76.64|114.5|45.01|105.4|140.7 CV(%)|6.4|5.3|4.9|7|6.8|5.7
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-29 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-29 has been precoated onto 96-well plates. Standards(NSO, G20-T200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-29 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-29 amount of sample captured in plate.
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human IL-29 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human IL-29 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.051|0.096|0.127|0.207|0.363|0.646|1.115|1.716
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-29 antibody|1ST0000-10|Lyophilized recombinant human IL-29 standard|10ng/tubex2AR1107|Biotinylated anti- human IL-29 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Interferon lambda-1 (IFNL1) |
Detection Range | 62.5pg/ml-4000pg/ml |
Alias | Cytokine ZCYTO21, IFN lambda 1, IFN-lambda-1, IFNL1, IL 29, IL-29, IL29, IL29_HUMAN, Interferon lambda 1, Interferon lambda-1, Interferon, lambda 1, Interleukin 29, Interleukin-29 |