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Boster
For quantitative detection of human IL-22 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL-22Protein name: Interleukin-22Gene Full Name: interleukin 22abbreviation: IL22Background: Interleukin-22(IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A. ID:Q9GZX6 ID:50616
Cytokine Zcyto18| IL 21| IL 22| IL D110| IL-10-related T-cell-derived-inducible factor| IL-22| IL-TIF| Il22| IL22_HUMAN| ILTIF| Interleukin 22| Interleukin-22| TIFa| TIFIL 23| zcyto18
Nativ and recombinant human IL-22
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, A34-I179
<10pg/ml
15.6pg/ml-1000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|74|312|611|87|386|695 Standard deviation|3.63|18.1|37.3|4.7|25.5|48 CV(%)|4.9|5.8|6.1|5.4|6.6|6.9
20-25
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-22 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-22 has been precoated onto 96-well plates. Standards(E.coli, A34-I179) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-22 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-22 amount of sample captured in plate.
Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human IL-22 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human IL-22 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.046|0.071|0.106|0.173|0.355|0.585|1.217|2.320
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-22 antibody|1ST0000-10|Lyophilized recombinant human IL-22 standard|10ng/tubex2AR1107|Biotinylated anti- human IL-22 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
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Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-22 (IL22) |
Detection Range | 15.6pg/ml-1000pg/ml |
Alias | Cytokine Zcyto18, IL 21, IL 22, IL D110, IL-10-related T-cell-derived-inducible factor, IL-22, IL-TIF, Il22, IL22_HUMAN, ILTIF, Interleukin 22, Interleukin-22, TIFa, TIFIL 23, zcyto18 |