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Boster
For quantitative detection of human IL-20 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL-20Protein name: Interleukin-20Gene Full Name: interleukin 20abbreviation: IL20Background: Interleukin-20(IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. By radiation hybrid analysis, the IL20 gene was mapped to chromosome 1q32, where it is tightly linked to the IL10, IL19, and MDA7 genes within a 195-kb region, the IL10 family cytokine cluster. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells. ID:Q9NYY1 ID:50604
Cytokine Zcyto10|Four alpha helix cytokine|IL-20|IL10D|IL20|IL20_HUMAN|Interleukin 20|Interleukin-20|MGC96907|UNQ852/PRO1801|ZCYTO10
Nativ and recombinant human IL-20
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, L25-E176
<10pg/ml
62.5pg/ml-4000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|548|1202|2279|623|1560|2533 Standard deviation|35.62|84.14|104.8|45.5|117|142 CV(%)|6.5|7|4.6|7.3|7.5|5.6
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-20 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-20 has been precoated onto 96-well plates. Standards(E.coli, L25-E176) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-20 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-20 amount of sample captured in plate.
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human IL-20 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human IL-20 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.134|0.176|0.203|0.259|0.377|0.667|1.200|1.989
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-20 antibody|1ST0000-10|Lyophilized recombinant human IL-20 standard|10ng/tubex2AR1107|Biotinylated anti- human IL-20 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-20 (IL20) |
Detection Range | 62.5pg/ml-4000pg/ml |
Alias | Cytokine like protein Zcyto7, Cytokine Zcyto7, IL 17B, IL 20, IL-17B, IL-20, IL17B, IL17B_HUMAN, IL20, Interleukin 17 beta, Interleukin 17B, Interleukin 20, Interleukin-17B, Interleukin-20, Neuronal interleukin 17 related factor, Neuronal interleukin-17-r |