Human IL-1ra ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human IL-1ra ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-1ra in human serum and plasma. An antibody specific for human IL-1ra was coated on a 96-well plate. Standards and samples are added to the wells and any IL-1ra present binds to the immobilized antibody. The wells are washed and biotinylated anti-human IL-1ra antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-1ra present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Human

detection target

interleukin 1 receptor antagonist(IL-1ra )
Background: IL-1ra /IL-1F3 (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. It binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. It blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.

alias

DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4

sensitivity

30 pg/ml

detection range

41.1-30000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P18510