Human IL-17RC PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-17RC in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL-17RC
Protein name: Interleukin-17 receptor C
Gene Full Name: interleukin 17 receptor C
abbreviation: IL17RC
Background: Interleukin-17 receptor C is a protein that in humans is encoded by the IL17RC gene. By genomic sequence analysis, IL17RC gene was mapped to chromosome 3p25.3-p24.1. This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. The biologic activity of IL-17 is dependent on a complex composed of IL-17RA and IL-17RC, suggesting a new paradigm for understanding the interactions between the expanded family of IL-17 ligands and their receptors.
ID:Q8NAC3
ID:84818

alias

IL 17 receptor C|IL 17RC|IL 17RL|IL17 receptor C|IL17F receptor|IL17RL|Interleukin 17 receptor C|Interleukin 17 receptor homolog|Interleukin 17 receptor like|Interleukin 17 receptor like protein

Origin

Nativ and recombinant human IL-17RC

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, L21-A454

sensitivity

<15pg/ml

detection range

250pg/ml-16000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|1.8|5|9.7|2.2|6.2|10.3
Standard deviation|0.101|0.32|0.427|0.132|0.428|0.546
CV(%)|5.6|6.4|4.4|6|6.9|5.3

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-17RC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17RC has been precoated onto 96-well plates. Standards(NSO, L21-A454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17RC amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 16000pg/ml, 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml human IL-17RC standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IL-17RC standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|250|500|1000|2000|4000|8000|16000 O.D.|0.003|0.080|0.141|0.283|0.575|1.068|1.729|2.234

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-17RC antibody|1
ST0000-20|Lyophilized recombinant human IL-17RC standard|20ng/tubex2
AR1107|Biotinylated anti- human IL-17RC antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4