Human IL-17RB PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-17RB in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL-17RB
Protein name: Interleukin-17 receptor B
Gene Full Name: interleukin 17 receptor B
abbreviation: IL17RB
Background: IL17RB, also known as CRL4, is a protein that in humans is encoded by the IL17RB gene. It is located on 3p21.1. IL17RB is produced by activated T cells exhibiting proinflammatory activities. The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E (IL25), but does not bind to IL17 (A) or IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. And it may play a role in controlling the growth and/or differentiation of hematopoietic cells.
ID:Q9NRM6
ID:55540

alias

CRL 4|CRL4|Cytokine receptor CRL4|EVI 27|EVI27|IL 17 receptor B|IL 17 receptor homolog 1|IL 17B receptor|IL 17RB|IL 17Rh1|IL17BR|IL17RB|IL17RH1|Interleukin 17 receptor B|Interleukin 17 receptor homolog 1|Interleukin 17 receptor homolog|Interleukin 17B receptor|MGC5245

Origin

Nativ and recombinant human IL-17RB

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, R18-K286

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|1.43|3.04|6.12|1.86|3.43|6.51
Standard deviation|0.053|0.137|0.324|0.106|0.233|0.469
CV(%)|3.7|4.5|5.3|5.7|6.8|7.2

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-17RB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17RB has been precoated onto 96-well plates. Standards(NSO, R18-K286) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17RB amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human IL-17RB standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IL-17RB standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.038|0.134|0.203|0.394|0.664|1.146|1.952|2.669

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-17RB antibody|1
ST0000-10|Lyophilized recombinant human IL-17RB standard|10ng/tube�2
AR1107|Biotinylated anti- human IL-17RB antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4