Human IL-17C PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-17C in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL-17C
Protein name: Interleukin-17C
Gene Full Name: interleukin 17C
abbreviation: IL17C
Background: IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.
ID:Q9P0M4
ID:27189

alias

CX2|Cytokine CX2|IL 17C|IL 21|IL-17C|IL17C|IL17C_HUMAN|Interleukin 17C|Interleukin-17C|MGC126884|MGC138401

Origin

Nativ and recombinant human IL-17C

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, H19-V197

sensitivity

<10pg/ml

detection range

125pg/ml-8000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|0.9|3.7|5.6|1.2|3.9|6.4
Standard deviation|0.042|0.189|0.252|0.064|0.261|0.371
CV(%)|4.7|5.1|4.5|5.3|6.7|5.8

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-17C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17C has been precoated onto 96-well plates. Standards(E.coli, H19-V197) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17C amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human IL-17C standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IL-17C standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|125|250|500|1000|2000|4000|8000 O.D.|0.035|0.109|0.179|0.286|0.543|0.970|1.466|2.203

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-17C antibody|1
ST0000-10|Lyophilized recombinant human IL-17C standard|10ng/tubex2
AR1107|Biotinylated anti- human IL-17C antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4