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Boster
For quantitative detection of human IL-17 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL-17Protein name: Interleukin-17AGene Full Name: interleukin 17Aabbreviation: IL17ABackground: IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri(HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa. ID:Q16552 ID:3605
CTLA 8| CTLA-8| CTLA8| Cytotoxic T lymphocyte associated antigen 8| Cytotoxic T lymphocyte associated protein 8| Cytotoxic T lymphocyte associated serine esterase 8| Cytotoxic T-lymphocyte-associated antigen 8| IL 17| IL 17A| IL-17| IL-17A| IL17_HUMAN| Il17a| Interleukin 17A| Interleukin-17A| Interleukin17| Interleukin17A
Nativ and recombinant human IL-17
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, I20-A155
<1pg/ml
31.2pg/ml-2000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|147|782|1434|158|825|1323 Standard deviation|6.91|39.1|64.53|12.5|70.95|95.3 CV(%)|4.7|5|4.5|7.9|8.6|7.2
20-25
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17 has been precoated onto 96-well plates. Standards(E.coli, I20-A155) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17 amount of sample captured in plate.
Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human IL-17 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human IL-17 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.034|0.106|0.177|0.293|0.555|0.978|1.511|2.113
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-17 antibody|1ST0000-10|Lyophilized recombinant human IL-17 standard|10ng/tubex2AR1107|Biotinylated anti- human IL-17 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <1pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-17A (IL17A) |
Detection Range | 31.2pg/ml-2000pg/ml |
Alias | CTLA 8, CTLA-8, CTLA8, Cytotoxic T lymphocyte associated antigen 8, Cytotoxic T lymphocyte associated protein 8, Cytotoxic T lymphocyte associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL 17, IL 17A, IL-17, IL-17A, I |