Human IL-16 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-16 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL-16
Protein name: Pro-interleukin-16
Gene Full Name: interleukin 16
abbreviation: IL16
Background: Interleukin 16(IL-16) is a cytokine that released by a variety of cells(including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor(LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.
ID:Q14005
ID:3603

alias

FLJ16806| FLJ42735| FLJ44234| HGNC:5980| HsT19289| IL-16| IL16| IL16_HUMAN| Interleukin 16| Interleukin 16 precursor| Interleukin-16| KIAA4048| LCF| Lymphocyte chemoattractant factor| mKIAA4048| Neuronal interleukin 16| NIL16| OTTHUMP00000185571| OTTHUMP00000190849| OTTHUMP00000190850| PrIL16| Pro interleukin 16| Prointerleukin 16

Origin

Nativ and recombinant human IL-16

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, P2-S130

sensitivity

<10pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|255|810|1389|292|735|1447
Standard deviation|13.52|48.6|68.1|19.86|54.39|82.48
CV(%)|5.3|6|4.9|6.8|7.4|5.7

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-16 has been precoated onto 96-well plates. Standards(E.coli, P2-S130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-16 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human IL-16 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IL-16 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.073|0.089|0.112|0.147|0.260|0.516|0.815|1.513

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-16 antibody|1
ST0000-10|Lyophilized recombinant human IL-16 standard|10ng/tubex2
AR1107|Biotinylated anti- human IL-16 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4