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Boster
For quantitative detection of human IL-13 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL-13Protein name: Interleukin-13Gene Full Name: interleukin 13abbreviation: IL13Background: Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells which is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4R alpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6 (STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. ID:P35225 ID:3596
Allergic rhinitis|ALRH|BHR 1|BHR1|Bronchial hyperresponsiveness 1 (bronchial asthma)|IL 13|IL-13|Il13|IL13_HUMAN|Interleukin 13|Interleukin-13|Interleukin13|MGC116786 MGC116788|MGC116789|NC30|P600
Nativ and recombinant human IL-13
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, G21-N132
<10pg/ml
15.6pg/ml-1000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|98|340|497|115|354|512 Standard deviation|4.02|10.88|19.38|8.28|19.82|29.7 CV(%)|4.1|3.2|3.9|7.2|5.6|5.8
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-13 has been precoated onto 96-well plates. Standards(E.coli, G21-N132) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-13 amount of sample captured in plate.
Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml human IL-13 standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. We recommend that each human IL-13 standard solution and each sample is measured in duplicate.
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.016|0.053|0.099|0.195|0.392|0.865|1.874|2.707
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-13 antibody|1ST0000-10|Lyophilized recombinant human IL-13 standard|10ng/tube�2AR1107|Biotinylated anti- human IL-13 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-13 (IL13) |
Detection Range | 15.6pg/ml-1000pg/ml |
Alias | Allergic rhinitis, ALRH, BHR 1, BHR1, Bronchial hyperresponsiveness 1 (bronchial asthma), IL 13, IL-13, Il13, IL13_HUMAN, Interleukin 13, Interleukin-13, Interleukin13, MGC116786 MGC116788, MGC116789, NC30, P600 |