Human IL-12 p70 ELISA Kit

Brand

Cell Sciences

Description

IL-12 is a heterodimeric 70 kDa glycoprotein (IL-12-p70) consisting of a 40 kDa subunit. It is secreted by peripheral lymphocytes after induction and is produced mainly by B-cells and to a lesser extent by T-cells. The most powerful inducers of IL-12 are bacteria, bacterial products, and parasites. IL-12 is produced after stimulation with Phorbol esters or Calcium ionophore by human B-lymphoblastoid cells. IL-12 activates NK-cells positive for CD56, and this activity is blocked by antibodies specific for TNF-alpha. IL-12 promotes specific allogenic CTL reactions. IL-12 synergizes also with anti-CD3 antibodies and with allogeneic stimulation in mixed lymphocyte cultures in inducing T-cell proliferation.The Cell Sciences Human IL-12(P70) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-12(P70) in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human IL-12(P70) coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12(P70) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-12 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12(P70) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

spezie

Human

Cross Reaction

Human

detection target

Human IL-12 p70
Antigen: IL12 (p70)

alias

CLMF, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, interleukin-12A, interleukin-12-alpha, natural killer cell stimulatory factor 1

sample size

1

pack size

PP

UNSPSC

41116126 and E