Human IL-10 R beta ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human IL-10 R beta ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-10 R beta in Human serum, plasma and cell culture media. An antibody specific for Human IL-10 R beta was coated on a 96-well plate. Standards and samples are added to the wells and any IL-10 R beta present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human IL-10 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-10 R beta present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Human

detection target

interleukin 10 receptor subunit beta(IL-10 R β)
Background: IL-10 R beta, also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

alias

CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2

sensitivity

2 pg/ml

detection range

4.1-3000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q08334