Human IL-10 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-10 in cell culture supernates, serum and plasma( heparin, EDTA, citrate).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL-10
Protein name: Interleukin-10
Gene Full Name: interleukin 10
abbreviation: IL10
Background: Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.
ID:P22301
ID:3586

alias

CSIF|Cytokine synthesis inhibitory factor|GVHDS|IL 10|IL-10|IL10|IL10_HUMAN|IL10A|Interleukin 10|Interleukin-10|Interleukin10|MGC126450|MGC126451|T cell growth inhibitory factor|TGIF

Origin

Nativ and recombinant human IL-10

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, S19-N178

sensitivity

<0.5pg/ml

detection range

7.8pg/ml-500pg/ml(cellculturesupernates)3.4pg/ml-250pg/ml(humanserum,plasma)

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|25|112|280|34|184|312
Standard deviation|1.35|5.38|8.68|2.55|14.54|20.9
CV(%)|5.4|4.8|3.1|7.5|7.9|6.7

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-10 has been precoated onto 96-well plates. Standards(E.coli, S19-N178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-10 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml human IL-10 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each human IL-10 standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|7.8|15.6|31.3|62.5|125|250|500 O.D.|0.023|0.080|0.126|0.226|0.424|0.749|1.388|2.493

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-10 antibody|1
ST0000-10|Lyophilized recombinant human IL-10 standard|10ng/tubex2
AR1107|Biotinylated anti- human IL-10 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4