Human IL-1 beta PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-1 beta in cell culture supernates, serum and plasma(heparin, EDTA, citrate).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL-1 beta
Protein name: Interleukin-1 beta
Gene Full Name: interleukin 1, beta
abbreviation: IL1B
Background: Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
ID:P01584
ID:3553

alias

Catabolin| Hematopoietin 1| IFN beta inducing factor| IL 1| IL 1 beta| IL 1B| IL-1 beta| IL1| IL1 BETA| IL1B| IL1B_HUMAN| IL1F2| Interleukin 1 beta| Interleukin 1 beta precursor| Interleukin-1 beta| LAF| OAF| Osteoclast activating factor| Preinterleukin beta| Pro interleukin 1 beta

Origin

Nativ and recombinant human IL-1 beta

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli,A117-S269

sensitivity

<0.15pg/ml

detection range

3.9pg/ml-250pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|19|41.2|116|27.5|61.3|179.1
Standard deviation|1.0|2.6|6.5|1.6|4.1|12.7
CV(%)|5.3|6.3|5.6|5.8|6.7|7.1

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-1 beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,A117-S269) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1 beta amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 16.5pg/ml, 7.8pg/ml, 3.9pg/ml human IL-1 betastandard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each human IL-1 betastandard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|3.9|7.8|15.6|31.2|62.5|125|250 O.D.|0.003|0.086|0.161|0.292|0.557|1.029|1.617|2.222

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-1 beta antibody|1
ST0000-1|Lyophilized recombinant human IL-1 beta standard|1ng/tubex2
AR1107|Biotinylated anti- human IL-1 beta antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4