Human IL-1 alpha PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-1 alpha in cell culture supernates, serum and plasma( heparin, EDTA, citrate).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL-1 alpha
Protein name: Interleukin-1 alpha
Gene Full Name: interleukin 1, alpha
abbreviation: IL1A
Background: Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18kDa.
ID:P01583
ID:3552

alias

Hematopoietin 1|IL 1 alpha|Il 1a|IL1|IL1 ALPHA|IL1A|IL1F1|Ilia|Interleukin 1 alpha|Interleukin 1 alpha precursor|Interleukin1 alpha|OTTHUMP00000203692|OTTMUSP00000016430|Preinterleukin 1|Pro interleukin 1|Pro interleukin 1 alpha|Prointerleukin 1|RP23-160G19.8

Origin

Nativ and recombinant human IL-1 alpha

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli,S113-A271

sensitivity

<0.5pg/ml

detection range

4.7pg/ml-300pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|54|147|221|48|139|234
Standard deviation|1.84|7.497|9.28|2.64|10.29|14.98
CV(%)|3.4|5.1|4.2|5.5|7.4|6.4

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S113-A271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1 alpha amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 300pg/ml, 150pg/ml, 75pg/ml, 37.5pg/ml, 18.8pg/ml, 9.4pg/ml, 4.7pg/ml human IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each human IL-1 alpha standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|4.7|9.4|18.8|37.5|75|150|300 O.D.|0.035|0.084|0.137|0.227|0.403|0.753|1.257|2.122

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-1 alpha antibody|1
ST0000-10|Lyophilized recombinant human IL-1 alpha standard|10ng/tubex2
AR1107|Biotinylated anti- human IL-1 alpha antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4