The Human IGFBP3 ELISA Pair Set is for the quantitative determination of Human IGFBP3. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IGFBP3 coated on a 96-well plate. Standards and samples are added to the wells, and any IGFBP3 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-IGFBP3 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of IGFBP3 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
IGFBP3Standard (antigene): Each vial contains 35 ng of recombinant IGFBP3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended. capture antibody type: 0.5 mg/mL of rabbit anti-IGFBP3 monoclonal antibody. Dilute to a working concentration of 0.5 µg/mL in CBS before coating. (Catalog: # 10430-R010)detection antibody type: 0.5 mg/mL rabbit anti-IGFBP3 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10430-R008)Lable: HRP
The minimum detectable dose of Human IGFBP3 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
The average concentration of IGFBP3 in 10 normal human serum is 2220.26+/-292.8 ng/mL ranging from 1578.63 to 2489.50 ng/mL.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of Human IGFBP3 was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Detection Range||31.25 pg/ml|