Human HPGD / 15-PGDH ELISA Kit

Brand

Sino Biological

short description

For the quantitative determination of Human HPGD / 15-PGDH concentration in cell lysate.

spezie

Human

natural samples' content determination

The concentration of Human HPGD detected in a MCF7 cell lysate was 46.87 ng/mL.The MCF7 cell lysate' total protein concentration was about 6.6 mg/mL .

Cross Reaction

This assay recognizes both recombinant and natural Human HPGD. Human CBR3, APP and mouse HPGD were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

detection target

HPGD
Antigene: 11205-H08E
capture antibody type: rabbit mAbdetection antibody type

sensitivity

4.83 pg/ml

detection range

31.25-2000 pg/ml

certificate

ISO9001-2008

pack size

96 Wells

storage and Stability

Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA

Principle of the Elisa

Solid Phase Sandwich ELISA

Kit Content

1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution