For the quantitative determination of Human HPGD / 15-PGDH concentration in cell lysate.
The concentration of Human HPGD detected in a MCF7 cell lysate was 46.87 ng/mL.The MCF7 cell lysate' total protein concentration was about 6.6 mg/mL .
This assay recognizes both recombinant and natural Human HPGD. Human CBR3, APP and mouse HPGD were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
HPGDAntigene: 11205-H08Ecapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||31.25-2000 pg/ml|
|Alias||HPGD / 15-PGDH|