For the quantitative determination of Human Fractalkine / CX3CL1 concentration in milk.
10 normal human milk were evaluated for the presence of human CX3CL1. Two samples measured less than the lowest standard, 7.82 pg/mL. Eight samples measured between 65.88 and 708.59 pg/mL.
This assay recognizes both recombinant and natural Human CX3CL1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CX3CL1Antigene: 10636-H08Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||7.82-500 pg/ml|
|Alias||Fractalkine / CX3CL1|