Human FGF-5 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human FGF-5 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of FGF-5 in Human serum, plasma and cell culture media. An antibody specific for Human FGF-5 was coated on a 96-well plate. Standards and samples are added to the wells and any FGF-5 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human FGF-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of FGF-5 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; cell growth;

specific against

Human

detection target

Fibroblast growth factor 5(FGF-5)
Background: FGF-5 continues to impact muscle post-natally where it is believed to function as a target-derived neurotrophic factor of skeletal muscle. In the nervous system, FGF-5 has been most often identified in neurons associated with the limbic system, notably in neurons of the olfactory bulb and pyramidal cells of the hippocampus. Hippocampal FGF-5 is suggested to serve as a neurotrophic and differentiative factor for cholinergic and serotonergic neurons projecting to this region.

alias

HBGF-5, Smag-82, TCMGLY

sensitivity

18 pg/ml

detection range

24.6-6000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P12034