The human Fetuin-A (AHSG / FETUA) ELISA Pair Set is for the quantitative determination of human Fetuin-A. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Fetuin-A ( AHSG / FETUA) coated on a 96-well plate. Standards and samples are added tothe wells, and any Fetuin-A (AHSG / FETUA) present binds to the immobilizedantibody. The wells are washed and a horseradish peroxidase conjugated rabbitanti-Fetuin-A monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof Fetuin-A (AHSG / FETUA) present in the sample. To end the enzyme reaction,the stop solution is added and absorbances of the microwell are read at 450 nm.
AHSGStandard (antigene): Each vial contains 24 ng of recombinant Fetuin-A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 800 pg/mL is recommended. capture antibody type: 0.5 mg/mL of mouse anti Fetuin-A monoclonal antibody. Dilute to a working concentration of 2.0 µg/mL in CBS before coating. (Catalog: # 10318-MM02)detection antibody type: 0.5 mg/mL of rabbit anti-Fetuin-A monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10318-R001)Lable: HRP
The minimum detectable dose of human Fetuin-A (AHSG / FETUA) was determined to be approximately 12.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of human Fetuin-A (AHSG / FETUA) was determined to be approximately 12.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Detection Range||12.5 pg/ml|
|Alias||Fetuin-A / AHSG / FETUA|