BioAim Scientific (Kanada)
The Bioaim Human Eotaxin-3 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Eotaxin-3 in Human serum, plasma and cell culture media. An antibody specific for Human Eotaxin-3 was coated on a 96-well plate. Standards and samples are added to the wells and any Eotaxin-3 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Eotaxin-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Eotaxin-3 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
C-C motif chemokine ligand 26(Eotaxin-3) Background: Eotaxin‑3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4. Eotaxin-3/CCL26 is chemotactic for eosinophils and PHA-activated T cells. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells.
IMAC, MIP-4a, MIP-4alpha, SCYA26, TSC-1
Serum, plasma, Cell culture supernatant
|Antigen Name||C-C motif chemokine ligand 26|
|Detection Range||28.7-7000 pg/ml|
|Alias||IMAC, MIP-4a, MIP-4alpha, SCYA26, TSC-1|