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BioAim Scientific (Kanada)
The Bioaim Human Eotaxin-3 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Eotaxin-3 in Human serum, plasma and cell culture media. An antibody specific for Human Eotaxin-3 was coated on a 96-well plate. Standards and samples are added to the wells and any Eotaxin-3 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Eotaxin-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Eotaxin-3 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
Human
C-C motif chemokine ligand 26(Eotaxin-3) Background: Eotaxin‑3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4. Eotaxin-3/CCL26 is chemotactic for eosinophils and PHA-activated T cells. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells.
IMAC, MIP-4a, MIP-4alpha, SCYA26, TSC-1
15 pg/ml
28.7-7000 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
Q9Y258
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 15 pg/ml |
Specific Against | Human |
Antigen Name | C-C motif chemokine ligand 26 |
Detection Range | 28.7-7000 pg/ml |
Alias | IMAC, MIP-4a, MIP-4alpha, SCYA26, TSC-1 |