Human EGFR ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human EGFR ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of EGFR in human serum and plasma. An antibody specific for human EGFR was coated on a 96-well plate. Standards and samples are added to the wells and any EGFR present binds to the immobilized antibody. The wells are washed and biotinylated anti-human EGFR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of EGFR present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cancer Biomarker; Cell adhesion; Inflammation;

specific against

Human

detection target

epidermal growth factor receptor (EGFR )
Background: The EGF R subfamily of receptor tyrosine kinases comprises four members: EGF R , ErbB2 , ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins. An extracellular ligand binding domain contains two cysteine-rich domains and a cytoplasmic domain. The cytoplasmic domain contains a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2; which results in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling. EGF R can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis.

alias

ERBB, ERBB1, HER1, NISBD2, PIG61, mENA

sensitivity

< 1 pg/ml

detection range

2.7-2000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P00533