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Boster
For quantitative detection of human DCR3 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human DCR3Protein name: Tumor necrosis factor receptor superfamily member 6BGene Full Name: tumor necrosis factor receptor superfamily, member 6b, decoyabbreviation: TNFRSF6BBackground: Decoy receptor 3 (Dcr3), also known as tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), TR6 and M68, is a soluble protein of the tumor necrosis factor receptor superfamily which inhibits Fas ligand-inducedapoptosis. This gene belongs to the tumor necrosis factor receptor superfamily. It acts as a decoy receptor that competes with death receptors for ligand binding. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation as well as to induce angiogenesis via neutralization of TL1A. Overexpression of this gene has been noted in various tumors e.g. gastrointestinal tract tumors, and it is located in a gene-rich cluster on chromosome 20, with other potentially tumor-related genes. ID:O95407 ID:8771
DcR3|Decoy Receptor 3|Decoy receptor for Fas ligand|DJ583P15.1.1|M68|M68E|OTTHUMP00000031583|TNF6B_HUMAN|TNFRSF6B|TR6|Tumor necrosis factor receptor superfamily member 6B|Tumor necrosis factor receptor superfamily member 6b decoy|Tumor necrosis factor receptor superfamily member 6b decoy|UNQ186/PRO212
Nativ and recombinant human DCR3
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
sf21, V24-H300
<10pg/ml
31.2pg/ml-2000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|45|177|705|51|193|833 Standard deviation|2.47|8.67|38.775|3.62|13.31|49.14 CV(%)|5.5|4.9|5.5|7.1|6.9|5.9
20-25
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human DCR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DCR3 has been precoated onto 96-well plates. Standards(sf21, V24 - H300) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DCR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DCR3 amount of sample captured in plate.
Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human DCR3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human DCR3 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.086|0.176|0.284|0.400|0.696|1.145|1.629|2.207
Catalog number|Description|Quantity-|96-well plate precoated with anti- human DCR3 antibody|1ST0000-10|Lyophilized recombinant human DCR3 standard|10ng/tube�2AR1107|Biotinylated anti- human DCR3 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Tumor necrosis factor receptor superfamily member 6B (TNFRSF6B) |
Detection Range | 31.2pg/ml-2000pg/ml |
Alias | DcR3, Decoy Receptor 3, Decoy receptor for Fas ligand, DJ583P15.1.1, M68, M68E, OTTHUMP00000031583, TNF6B_HUMAN, TNFRSF6B, TR6, Tumor necrosis factor receptor superfamily member 6B, Tumor necrosis factor receptor superfamily member 6b decoy, Tumor necrosi |