For the quantitative determination of Human Cystatin C / CST3 concentration in serum, cell culture supernates, and urine.
The average concentration of CST3 in 10 normal human serum is 66.98 +/- 24.39 ng/mL ranging from 41.39 to 113.36 ng/mL. The average concentration of CST3 in 10 normal human urine is 4.30 +/- 2.50 ng/mL ranging from 0.67 to 9.50 ng/mL. HepG2 cells (0.2 x 10E5 cells/mL) were cultured 3 days. An aliquot of the cell culture supernate was removed, assayed for levels of natural CST3, and measured 15.58 ng/mL.
This assay recognizes both recombinant and natural Human CST3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CST3Antigene: 10439-H08Hcapture antibody type: mouse mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Antigen Name||Cystatin C|
|Detection Range||3.91-250 pg/ml|
|Alias||Cystatin C / CST3|