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Boster
For quantitative detection of human CSF3R in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human CSF3RProtein name: Granulocyte colony-stimulating factor receptorGene Full Name: colony stimulating factor 3 receptor (granulocyte)abbreviation: CSF3RBackground: The granulocyte colony-stimulating factor receptor (G-CSF-R), also known as CD114 (Cluster of Differentiation 114), is a protein that in humans is encoded by the CSF3R gene. It is mapped to 1p35-p34.3. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). And the G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. In addition, GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, andErk1/2. ID:Q99062 ID:1441
CD 114|CD114|CD114 antigen|Colony stimulating factor 3 receptor (granulocyte)|Colony stimulating factor 3 receptor|CSF 3R|CSF3R| CSF3R_HUMAN|Csfgr|G CSF R|G-CSF receptor|G-CSF-R|GCSFR|Granulocyte colony stimulating factor receptor|Granulocyte colony-stimulating factor receptor|OTTHUMP00000009703|OTTHUMP00000009704|OTTHUMP00000009705
Nativ and recombinant human CSF3R
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
NSO, E25-P621
<10pg/ml
31.2pg/ml-2000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|236|819|1237|326|928|1528 Standard deviation|12.51|50|59.4|21.19|66.82|84.04 CV(%)|5.3|6.1|4.8|6.5|7.2|5.5
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human CSF3R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CSF3R has been precoated onto 96-well plates. Standards(NSO, E25-P621) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CSF3R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CSF3R amount of sample captured in plate.
Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human CSF3R standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human CSF3R standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.041|0.133|0.177|0.356|0.570|1.023|1.898|2.402
Catalog number|Description|Quantity-|96-well plate precoated with anti- human CSF3R antibody|1ST0000-10|Lyophilized recombinant human CSF3R standard|10ng/tube�2AR1107|Biotinylated anti- human CSF3R antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Granulocyte colony-stimulating factor receptor (CSF3R) |
Detection Range | 31.2pg/ml-2000pg/ml |
Alias | CD 114, CD114, CD114 antigen, Colony stimulating factor 3 receptor (granulocyte), Colony stimulating factor 3 receptor, CSF 3R, CSF3R, CSF3R_HUMAN, Csfgr, G CSF R, G-CSF receptor, G-CSF-R, GCSFR, Granulocyte colony stimulating factor receptor, Granulocyte |