The Human Complement C5a ELISA Pair Set is for the quantitative determination of Human Complement C5a . The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Complement C5a coated on a 96-well plate. Standards and samples are added to the wells, and any Human Complement C5a present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human Complement C5a polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Complement C5a present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
C5Standard (antigene): Each vial contains 100 ng of recombinant Human Complement C5a . Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended. capture antibody type: 1 mg/mL of mouse anti-Human Complement C5a monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 µg/mL in PBS before coating. (Catalog: # 10604-MM06)detection antibody type: 0.2 mg/mL of rabbit anti-Human Complement C5a polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4°C;). Dilute to working concentration of 0.25 µg/mL in detection antibody dilution buffer before use.Lable: HRP
The minimum detectable dose of Human Complement C5a was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of Human Complement C5a was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Antigen Name||Complement C5a|
|Detection Range||31.25 pg/ml|