Human COMP / TSP5 ELISA Pair Set

Brand

Sino Biological

short description

The human COMP / TSP5 ELISA Pair Set is for the quantitative determination of human COMP. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for COMP /TSP5 coated on a 96-well plate. Standards and samples are added to the wells,and any COMP / TSP5 present binds to the immobilized antibody. The wells arewashed and a biotinylated rabbit anti-COMP polyclonal antibody is then added,producing an antibody-antigen-antibody "sandwich". To produces color inproportion to the amount of COMP / TSP5 present in the sample strepavidin-HRPand TMB substrate solution are loaded. The absorbances of the microwell areread at 450 nm.

spezie

Human

detection target

COMP
Standard (antigene): Each vial contains 60 ng of recombinant COMP. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
capture antibody type: 0.5 mg/mL of mouse anti-COMP monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 10173-MM02)
detection antibody type: Each vial contains 120 µg biotinylated rabbit anti-COMP polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 2 µg/mL in detection antibody dilution buffer before use.
Lable: Biotin

sensitivity

The minimum detectable dose of human COMP / TSP5 was determined to be approximately 39.1 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

39.1 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4°C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.