Human CD97 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD97 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human CD97
Protein name: CD97 antigen
Gene Full Name: adhesion G protein-coupled receptor E5
abbreviation: ADGRE5
Background: Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.
ID:P48960
ID:976

alias

CD 97|CD_antigen=CD97|CD97|CD97 antigen|CD97 antigen subunit beta|CD97 molecule|CD97_HUMAN|Leukocyte antigen CD97|Seven span transmembrane protein|Seven transmembrane helix receptor|Seven transmembrane heterodimeric receptor associated with inflammation|TM7LN 1|TM7LN1

Origin

Nativ and recombinant human CD97

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, Q21-Q398

sensitivity

<10pg/ml

detection range

125pg/ml-8000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|199|894|2165|201|715|3013
Standard deviation|11.54|52.74|134.23|12.86|47.19|213.92
CV(%)|5.8|5.9|6.2|6.4|6.6|7.1

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD97 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD97 has been precoated onto 96-well plates. Standards (NSO, Q21-Q398) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD97 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD97 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human CD97 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human CD97 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|125|250|500|1000|2000|4000|8000 O.D.|0.004|0.102|0.175|0.333|0.559|1.021|1.672|2.054

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD97 antibody|1
ST0000-10|Lyophilized recombinant human CD97 standard|10ng/tube�2
AR1107|Biotinylated anti- human CD97 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4