Human CD40 Ligand ELISA Kit

Brand

Cell Sciences

Description

CD40L is expressed predominantly on activated CD4+T lymphocytes, and also found in other types of cells, like NK cells, mastcells, basophils and eosinophils. CD40 is expressed on all B-lymphocytes during various stages of development, activated T-cellsand monocytes, follicular dendritic cells, thymic epithelial cells, and various carcinoma cell lines. CD40L mediates a range ofactivities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Igsecretion and memory generation. CD40 ligation has been shown to induce cell death by apoptosis in transformed cells ofmesenchymal and epithelial origin.The Cell Sciences Human CD40L ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbentassay for the quantitative measurement of human CD40L in serum, plasma (EDTA or heparin), cell culture supernatants and urine.This assay employs an antibody specific for human CD40L coated on a 96-well plate. Standards and samples are pipetted into thewells and CD40L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylatedanti-human CD40L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipettedto the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to theamount of CD40L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

spezie

Human

Cross Reaction

Human

detection target

Human CD40 Ligand
Antigen: CD40 Ligand/CD154

alias

CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40

sample size

1

pack size

PP

UNSPSC

41116126 and E