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Boster
For quantitative detection of human CD30 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human CD30Protein name: Tumor necrosis factor receptor superfamily member 8Gene Full Name: tumor necrosis factor receptor superfamily, member 8abbreviation: TNFRSF8Background: CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma. ID:P28908 ID:943
CD 30|CD153|CD30|CD30 antigen|CD30L receptor|Cytokine receptor CD30|D1S166E|KI 1|Ki 1 antigen|Ki-1 antigen|KI1|Lymphocyte activation antigen CD30|TNFRSF 8|TNFRSF8|TNFRSF8|TNR8_HUMAN|Tumor Necrosis Factor Receptor Superfamily Member 8|Tumor Necrosis Factor Receptor Superfamily Member 8
Nativ and recombinant human CD30
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
NSO, F19-K379
<10pg/ml
156pg/ml-10000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(ng/ml)|0.48|1.3|5.2|0.52|2.4|6.3 Standard deviation|0.02|0.06|0.29|0.03|0.14|0.4 CV(%)|3.3|4.5|5.6|4.9|5.8|6.2
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human CD30 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD30 has been precoated onto 96-well plates. Standards(NSO, F19-K379) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD30 amount of sample captured in plate.
Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml human CD30 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human CD30 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.091|0.145|0.231|0.379|0.547|1.009|1.503|2.241
Catalog number|Description|Quantity-|96-well plate precoated with anti- human CD30 antibody|1ST0000-10|Lyophilized recombinant human CD30 standard|10ng/tube�2AR1107|Biotinylated anti- human CD30 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Tumor necrosis factor receptor superfamily member 8 (TNFRSF8) |
Detection Range | 156pg/ml-10000pg/ml |
Alias | CD 30, CD153, CD30, CD30 antigen, CD30L receptor, Cytokine receptor CD30, D1S166E, KI 1, Ki 1 antigen, Ki-1 antigen, KI1, Lymphocyte activation antigen CD30, TNFRSF 8, TNFRSF8, TNFRSF8, TNR8_HUMAN, Tumor Necrosis Factor Receptor Superfamily Member 8, Tumo |