Human CD157 / BST1 ELISA Pair Set

Brand

Sino Biological

short description

The human BST-1 / CD157 ELISA Pair Set is for the quantitative determination of human BST-1 / CD157. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for BST-1 / CD157 coated on a 96-well plate. Standards and samples are added to the wells, and any BST-1 / CD157 present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-BST-1 / CD157 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of BST-1 / CD157 present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.

spezie

Human

detection target

BST1
Standard (antigene): Each vial contains 15 ng of recombinant BST1. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1 ng/mL is recommended.
capture antibody type: 0.5 mg/mL of mouse anti-BST1 monoclonal antibody. Dilute to a working concentration of 1 µg/mL in CBS before coating. (Catalog: # 10060-MM05)
detection antibody type: 0.3 mg/mL of biotinylated rabbit anti-BST1 polyclonal antibody. Dilute to a working concentration of 0.2 µg/mL in detection antibody dilution buffer before use.
Lable: Biotin

sensitivity

The minimum detectable dose of human BST-1 / CD157 was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

15.6 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4°C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.