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Human CD112 / Nectin-2 / PVRL2 ELISA Pair Set

Product Code: SEK10005

Verfügbarkeit: Auf Lager

Special Price 218,82 €

Regulärer Preis: 230,34 €

Zzgl. 19% USt.

Human CD112 / Nectin-2 / PVRL2 ELISA Pair Set

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Special Price 218,82 €

Regulärer Preis: 230,34 €

Zzgl. 19% USt.

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Beschreibung

Details

Human CD112 / Nectin-2 / PVRL2 ELISA Pair Set

Brand

Sino Biological

short description

The Human CD112 / Nectin-2 ELISA Pair Set is for the quantitative determination of Human CD112 / Nectin-2. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD112 / Nectin-2 coated on a 96-well plate. Standards and samples are added to the wells, and any CD112 / Nectin-2 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD112 / Nectin-2 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD112 / Nectin-2 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

Human

detection target

PVRL2
Standard (antigene): Each vial contains 50 ng of recombinant CD112. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
capture antibody type: 0.25 mg/mL of rabbit anti-CD112 monoclonal antibody, Dilute to a working concentration of 2.0 µg/mL in CBS before coating. (Catalog: # 10005-R005)
detection antibody type: 0.5 mg/mL rabbit anti-CD112 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.1 µg/mL in detection antibody dilution buffer before use.
Lable: HRP

sensitivity

The minimum detectable dose of Human CD112 / Nectin-2 was determined to be approximately 15.625 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

15.625 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.

Weitere Informationen

Weitere Informationen

Hersteller Sino Biological
Herstellerland China
Sensitivity The minimum detectable dose of Human CD112 / Nectin-2 was determined to be approximately 15.625 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Specific Against Human
Antigen Name CD112
Detection Range 15.625 pg/ml
Zertifikat ISO9001-2008
Alias CD112 / Nectin-2 / PVRL2
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