Human Cathepsin L ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Cadherin L ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Cadherin L in Human serum, plasma and cell culture media. An antibody specific for Human Cadherin L was coated on a 96-well plate. Standards and samples are added to the wells and any Cadherin L present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Cadherin L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Cadherin L present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis;

specific against

Human

detection target

Cathepsin L(Cathepsin L)
Background: Cathepsin L is a lysosomal cysteine protease expressed in most eukaryotic cells. Cathepsin L is known to hydrolyze a number of proteins, including the proform of urokinase-type plasminogen activator, which is activated by Cathepsin L cleavage. Cathepsin L has also been shown to proteolytically inactivate a1-antitrypsin and secretory leucoprotease inhibitor. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis.

alias

CATL1, MEP, CTSL

sensitivity

15 pg/ml

detection range

24.6-6000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P07711