Human and Mouse AKT Pathway Phosphorylation Array C1

Brand

Raybiotech

short description

RayBio® C-Series Human and Mouse AKT Pathway Phosphorylation Array C1. Detects 18 phosphorylated human and mouse proteins. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Intended Use Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process;

specific against

Human, Mouse

detection target

Akt (P-Ser473) (PKB (P-Ser473)), AMPKa (P-Thr172), BAD (P-Ser112), 4E-BP1 (P-Thr36), ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187), GSK3a (P-Ser21), GSK3b (P-Ser9), mTOR (P-Ser2448), p27 (P-Thr198), P53 (P-Ser15), P70S6K (P-Thr421/Ser424), PDK1 (P-S241), PRAS40 (P-Thr246), PTEN (P-Ser380), Raf-1 (Ser301), RPS6 (P-Ser235/Ser236), RSK1 (P-Ser380), RSK2 (P-Ser386)

certificate

GMP/ISO

pack size

2, 4 and 8 Sample Kit

storage and Stability

For best results, store the entire kit frozen a -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer a -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Principle of the Elisa

AKT Arrays - Sandwich-based

Kit Content

• Human/Mouse AKT Pathwayway Membranes
• Blocking Buffer
• Wash Buffer 1
• Wash Buffer 2
• Detection Antibody Cocktail
• HRP-Conjugated anti-IgG
• Detection Buffer C
• Detection Buffer D
• Lysis Buffer
• 8-Well Incubation Tray
• Plastic Sheets
• Array Templates
• Manual