Human Afamin PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human Afamin in cell culture supernates, serum and urine.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

alias

Afamin | Afm | ALB 2 | ALB2 | ALBA | ALF | Alpha albumin | Alpha-albumin | Alpha-Alb | P43652

Origin

Natural and recombinant human Afamin

Immunogene

NSO, L22-N599

sensitivity

<10pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|175|742|1486|208|914|1658 Standard deviation|8.05|42.29|101.05|8.74|50.27|122.69 CV(%)|4.6|5.7|6.8|4.2|5.5|7.4

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Bosterﾒs human Afamin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Afamin has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: L22-N599) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Afamin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Afamin amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human Afamin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or urine to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human Afamin standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.030|0.149|0.251|0.480|0.751|1.261|1.806|2.289

Kit Content

Catalog number|Description|Quantity EK1487-CAP|96-well plate precoated with anti- human Afamin antibody|1 EK1487-ST|Lyophilized recombinant human Afamin standard|2ng/tube�2 EK1487-DA|Biotinylated anti- human Afamin antibody|130?l(dilution 1:100) AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130?l(dilution 1:100) AR1106-1|Sample diluent buffer|30ml AR1106-2|Antibody diluent buffer|12ml AR1106-3|ABC diluent buffer|12ml AR1104|TMB color developing agent|10ml AR1105|TMB stop solution|10ml PLA-SEA|Adhesive cover|4