Human 6Ckine ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human 6Ckine ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of 6Ckine in Human serum, plasma and cell culture media. An antibody specific for Human 6Ckine was coated on a 96-well plate. Standards and samples are added to the wells and any 6Ckine present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human 6Ckine antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of 6Ckine present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; inflammation;

specific against

Human

detection target

C-C motif chemokine 21(6Ckine)
Background: CCL21, also known as 6Ckine, TCA-4, SLC, Exodus-2, and A21, is a homeostatic chemokine that signals through CCR7 or CXCR3. CCL21 is constitutively presented on initial lymphatic vessels, high endothelial venules (HEV), and lymph node dendritic cells (DC) where it promotes the docking of DC to lymphatic vessels and the retention of T cells by lymph node DC, resulting in T cell priming for activation. Its upregulation during chronic inflammation or tissue damage promotes fibrosis, inflammatory cytokine production, and neuropathic pain. The soluble chemokine is elevated in rheumatoid arthritis synovial fluid and in the serum of coronary artery disease patients.

alias

6Ckine, CKb9, ECL, SCYA21, SLC, TCA4

sensitivity

20 pg/ml

detection range

32.8-8000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

O00585