High (to 0.5 U/mL) Heparin coated 96 well plate

Brand

Lifespan Technologies

short description

Heparin is a glycosaminoglycan with alternating uronic acid and aminoglycoside units. It is an anticoagulant used either in its native unfractionated form (UFH) MW ~16 kD or in various partially depolymerized forms (LMWH) of 4-8 kD. The heparin-ELISA product number K-2300 is a quantitative enzyme-linked assay designed for the in vitro measurement of unfractionated heparin levels in low protein content fluids such as buffer or urine. This assay measures heparin directly using a heparin binding protein which has been conjugated to HRP. The heparin-ELISA is a competitive assay in which the colorimetric signal is inversely proportional to the amount of heparin present in the sample. Samples to be assayed are first mixed with the Detector-Enzyme Conjugate in wells of the heparin coated plate. Heparin in the sample competes with heparin bound to the plate for binding of the Detector-Enzyme Conjugate. The concentration of heparin in the sample is determined using a standard curve of known amounts of heparin. For best results, the heparin used for the standard curve should match the type of heparin being assayed.
Multichannel Pipetter Absorbance microplate reader Plate Cover UFH Standard from USP reference or your heparin Standard Diluent, normal saline (154 mM NaCl)

spezie

Human

detection target

High Heparin

pack size

1x96 Wells

storage and Stability

Kit can be stored unopened at 4°C for up to six months. Opened and reconstituted solutions can be used for up to one week when stored at 4°C. The TMB solution should be protected from light. Reconstituted detector enzyme conjugate should be used immediately or aliquotted and stored at -80°C

Kit Content

Heparin-coated 96-well plate Detector -Enzyme Conjugate Conjugate Diluent TMB Solution Stop Solution, 0.5M H2SO4 Wash Concentrate 10X, (dilute 1 part plus 9 parts dH2O to make TBS-0.05% TWEEN 20)