Product Description:

Cell Biolabsﾒ HiF-1 Alpha DNA Binding Activity Assay Kit is an immunoassay developed for rapid detection of activated HiF-1 in any protein sample. Active HiF-1 complex is captured on a double stranded oligonucleotide containing an HRE that is attached to the well. HiF-1 Alpha is then detected with an anti-HiF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HiF-1 Alpha from human, mouse, or rat. The HiF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method.

Product Line:

Cell Based Assays

Product Classification:

Cell Based Assays->Cell Health Assays >Hypoxia Assays

Shipping Temp:

Room Temp

Storage Temp:

Upon receipt, store the Biotinylated Double Stranded DNA and Anti-HiF-1 Alpha Antibody at -20C. Store all other components at 4C.