Haptoglobin (Sheep) ELISA Kit : 96 wells (OKIA00188)

Brand

AVIVA Systems Biology

short description

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Haptoglobin (Hp) present in samples reacts with the anti-Hp antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hp in the test sample. The quantity of Hp in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Immunoperoxidase Assay for Determination of Haptoglobin in Sheep Samples

spezie

Sheep

detection target

Haptoglobin (Sheep)
description of target: SHEEP HAPTOGLOBIN
gen symbol: HP
Gene ID: 101102413

alias

Haptoglobin, HP, Zonulin

sensitivity

5.0465 ng/mL

pack size

96 Wells

reconstitution and storage

Component Description Preparation Storage Stability ELISA Micro Plate, antibody coated One plate of 12 removable 8 well strips, antibody coated Ready to use as supplied. 4-8 The plate strips are stable until the expiration date if stored correctly. Enzyme Conjugated Detection Antibody One vial of 150uL of 100X Horseradish Peroxidase Conjugated antibody in a stabilizing buffer Dilute 1/100 immediately prior to use. 4-8 in the dark The working conjugate solution should be diluted immediately prior to use. The 100X conjugate is stable until the expiration date. Calibrator One vial of lyophilized calibrator Refer to the Certificate of Analysis (CoA). 4-8 for lyophilized calibrator. Aliquoted and frozen if re-constituted. Avoid multiple freeze-thaw cycles. The working standard solutions should be prepared immediately prior to use. Diluent Concentrate One 50 mL bottle of 5X diluent buffer Dilute 1/5 to make 1X working solution. 4-8C for both 1X working solution and 5X diluent concentrate The 1X working solution is stable for at least one week from the date of preparation. The 5X concentrate is stable until the expiration date. Wash Solution Concentrate One 50 mL bottle of 20X wash solution Dilute 1/20 to make 1X working solution. 4-8C for both 1X working solution and 20X diluent concentrate The 1X working solution is stable for at least one week from the date of preparation. The 20X concentrate is stable until the expiration date. Chromogen-Substrate Solution One bottle of 12 mL 3,3',5,5'tetramethylbenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. Ready to use as supplied 4-8 C in the dark Protect from light. The Substrate Solution is stable until the expiration date. STOP Solution WARNING: Avoid Contact with Skin One 12 mL bottle of 0.3 M sulfuric acid. Ready to use as supplied 4-8C The Stop Solution is stable until the expiration date.

Weitere Informationen

Weitere Informationen

Menge	96 Wells
Hersteller	AVIVA Systems Biology
Herstellerland	USA
Sensitivity	5.0465 ng/mL
Specific Against	Salmon
Antigen Name	Haptoglobin (Sheep)