Free Beta-Subunit of Human Chorionic Gonadotropin (Free beta-hCG)

Brand

GENWAY

Overview

Intended Use: For the quantitative determination of free beta subunit of human chorionic gonadotropin (free ?-hCG) concentration in human serum. Introduction: Human Chorionic Gonadotropin (hCG) is a glycoprotein hormone normally produced by placenta during pregnancy. The hormone is present in blood and urine around seven to thirteen days following implantation of the fertilized ovum. Structurally intact hCG molecules consist of two non-covalently linked polypeptide subunits the alpha and beta chain subunits. Measurement of intact hCG and of the alpha subunit of hCG appears to give similar results in blood and urine but not the levels of beta subunit. In the normal second-trimester maternal sera the level of intact hCG range from 20 000 mIU/ml to 50 000 mIU/ml (1 ng = 15 mIU). In contrast the levels of either free ?- or free ?-hCG are on average one half of 1% of hCG levels. hCG and the free subunits appear not to be useful as serological markers for nontrophoblastic tumors; however the absolute increase of ?-hCG level in choriocarcinoma samples clearly differentiates it from normal pregnancyPrinciple of the test: The free ?-hCG ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. 1 The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the free ?-hCG. Mouse monoclonal anti-free-?-hCG antibody is used for solid phase immobilization (on the microtiter wells). A goat anti whole hCG antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react sequentially with the two antibodies resulting in the free ?-hCG molecules being sandwiched between the solid phase and enzyme-linked antibodies. After two separate 30 minute incubations at 37 C the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of ?-hCG is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. Limitation of the Test: 1. Reliable and reproducible results will be obtained when the assay procedure is carried out with a complete understanding of the package insert instructions and will adherence to good laboratory practice. 2. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings. 3. Serum samples demonstrating gross lipemia gross hemolysis or turbidity should not be used with this test. Product Name: Free Beta-Subunit of Human Chorionic Gonadotropin (Free beta-hCG) Function: Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. Subunit: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin lutropin follitropin and gonadotropin. Subcellular Location: Secreted. Tissue Specificity: Placenta. Developmental Stage: Made by the first trimester placenta. Pharmaceutical: Available under the names Novarel (Ferring) and Profasi (Serono). Used as adjunctive therapy in the treatment of obesity. There is no substantial evidence that it increases weight loss beyond that resulting from caloric restriction that it causes a more attractive or \'normal\' distribution of fat or that it decreases the hunger and discomfort associated with calorie-restricted diets. Similarity: Belongs to the glycoprotein hormones subunit beta family.

spezie

Human

detection target

Free Beta-Subunit of Chorionic Gonadotropin (Free beta-hCG)

pack size

1x96 Wells