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Enzyme-linked immunosorbent assay for Antigen Detection.Research focus: Cytokine; Tumor immunity; Infection immunity; ;
Homo sapiens (Human)
This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b).
No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.
Interleukin 1 Beta
IL1-B; IL1-Beta; IL1F2; IL-1?; Interleukin-1 Family Member 2; Catabolin
The minimum detectable dose of this kit is typically less than 5.8 pg/mL
15.6-1000 pg/mL
ISO 9001:2008, ISO 13485:2003
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
48T, 96T, 5x96T, 10x96T
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
3h
All the kits should stored according to the manual. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree upon receipt while the others should be at 4 degree.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Double-antibody SandwichTest principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Beta (IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Beta (IL1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Pre-coated, ready to use 96-well strip plate: 1Plate sealer for 96 wells: 4Standard: 2 Standard Diluent: 1x20mL Detection Reagent A: 1x120µ L Assay Diluent A: 1x12mL Detection Reagent B: 1x120µ L Assay Diluent B: 1x12mL TMB Substrate: 1x9mL Stop Solution: 1x6mL Wash Buffer (30 x concentrate): 1x20mL Instruction manual: 1
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.
P01584
A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches; Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1?) attenuates traumatic brain injury in rats.; Experimental peri-implant mucositis at different implant surfaces.; Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical Trial; MRTF-A steers an epigenetic complex to activate endothelin-induced pro-inflammatory transcription in vascular smooth muscle cells; GYY4137, a hydrogen sulfide?releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor?kappa B and mitogen?activated protein kinases in Coxsackie virus B3?infected rat cardiomyocytes.; Relationship between serum secreted frizzled-related protein 4 levels and the first-phase of glucose-stimulated insulin secretion in individuals with different glucose tolerance; MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1; MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1; The immunomodulating effect of seminal plasma on T cells; Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery; Low Shear Stress Induced HMGB1 Translocation and Release via PECAM-1/PARP-3 Pathway to Induce Inflammation Response; Inflammosome in the human endometrium: further step in the evaluation of the モmaternal sideヤ; ??????????????????????????????????; Screening the anti-gout traditional herbs from TCM using an in vitro method;
Hersteller | Cloud-Clone |
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Herstellerland | China |
Sensitivity | The minimum detectable dose of this kit is typically less than 6.3 pg/mL |
Specific Against | Human |
Antigen Name | Interleukin 1 Beta (IL1b) |
Detection Range | 15.6-1000 pg/mL |
Zertifikat | ISO 9001:2008, ISO 13485:2003 |
Referenz | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Id |
Alias | IL1-B/ IL1-Beta/ IL1F2/ IL-1?/ Interleukin-1 Family Member 2/ Catabolin |