ELISA Kit for Interleukin 1 Beta (IL1b)

Brand

Cloud-Clone

short description

Enzyme-linked immunosorbent assay for Antigen Detection.
Research focus: Cytokine; Tumor immunity; Infection immunity; ;

spezie

Mus musculus (Mouse)

Cross Reaction

This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b).
No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.

detection target

Interleukin 1 Beta

alias

IL1-B; IL1-Beta; IL1F2; IL-1?; Interleukin-1 Family Member 2; Catabolin

sensitivity

The minimum detectable dose of this kit is typically less than 6.4 pg/mL

detection range

15.6-1000 pg/mL

certificate

ISO 9001:2008, ISO 13485:2003

sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

pack size

48T, 96T, 5x96T, 10x96T

Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Assay time

3h

storage and Stability

All the kits should stored according to the manual.
The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree upon receipt while the others should be at 4 degree.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Principle of the Elisa

Double-antibody Sandwich
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Beta (IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Beta (IL1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Kit Content

Pre-coated, ready to use 96-well strip plate: 1
Plate sealer for 96 wells: 4
Standard: 2
Standard Diluent: 1x20mL
Detection Reagent A: 1x120µ L
Assay Diluent A: 1x12mL
Detection Reagent B: 1x120µ L
Assay Diluent B: 1x12mL
TMB Substrate: 1x9mL
Stop Solution: 1x6mL
Wash Buffer (30 x concentrate): 1x20mL
Instruction manual: 1

Assay procrdure summery

1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.

Uniport ID

P10749

Reference

Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice; Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice; Ixora coccinea down Regulate the Inflammation-Associated Prostaglandins (PGE-2), Nitric Oxide (NO) and Cytokines Production in LPS-Stimulated RAW 264.7 Macrophage; Ixora coccinea down Regulate the Inflammation-Associated Prostaglandins (PGE-2), Nitric Oxide (NO) and Cytokines Production in LPS-Stimulated RAW 264.7 Macrophage; Anti-inflammatory Effect of Euphorbia hirta Leaf Extract in Rats and Modulation of Inflammation-Associated Prostaglandins (PGE-2) and Nitric Oxide (NO) Expression in RAW264.7 Macrophage; Anti-inflammatory Effect of Euphorbia hirta Leaf Extract in Rats and Modulation of Inflammation-Associated Prostaglandins (PGE-2) and Nitric Oxide (NO) Expression in RAW264.7 Macrophage; Ginsenosides Rg1 from Panax ginseng: A Potential Therapy for Acute Liver Failure Patients?; Ginsenosides Rg1 from Panax ginseng: A Potential Therapy for Acute Liver Failure Patients?; Valproic acid protects septic mice from renal injury by reducing the inflammatory response; Valproic acid protects septic mice from renal injury by reducing the inflammatory response; Protective effects of astaxanthin against ischemia/reperfusion induced renal injury in mice; Protective effects of astaxanthin against ischemia/reperfusion induced renal injury in mice; Roles of lipoxin A4 receptor activation and anti-interleukin-1? antibody on the toll-like receptor 2/mycloid differentiation factor 88/nuclear factor-?B pathway in airway inflammation induced by ovalbumin; Effect of SecinH3 on lung injury induced by sepsis of rats; Epigallocatechin-3-Gallate Attenuates Unilateral Ureteral Obstruction-Induced Renal Interstitial Fibrosis in Mice; Epigallocatechin-3-Gallate Attenuates Unilateral Ureteral Obstruction-Induced Renal Interstitial Fibrosis in Mice; MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1; MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1; Aggregatibacter actinomycetemcomitans Invasion Induces Interleukin-1? Production Through Reactive Oxygen Species and Cathepsin B; Construction of recombinant Mip-FlaA dominant epitope vaccine against Legionella pneumophila and evaluation of the immunogenicity and protective immunity; Construction of recombinant Mip-FlaA dominant epitope vaccine against Legionella pneumophila and evaluation of the immunogenicity and protective immunity; Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in mice; Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4; Bexarotene protects against traumatic brain injury in mice partially through apolipoprotein E; A chimeric protein composed of the binding domains ofClostridium perfringensﾠphospholipase C andﾠTrueperella pyogenesﾠpyolysin induces partial immunoprotection in a mouse model; Ethyl pyruvateﾠattenuatedﾠcoxsackievirusﾠB3-inducedﾠacuteﾠviralﾠmyocarditisﾠbyﾠsuppressionﾠofHMGB1/RAGE/NF-?Bﾠpathway.; Carcinoembryonic antigen related cellular adhesion molecule 1 alleviates dextran sulfate sodium-induced ulcerative colitis in mice;