ELISA Kit for Interleukin 1 Beta (IL1b)

Brand

Cloud-Clone

short description

Enzyme-linked immunosorbent assay for Antigen Detection.
Research focus: Cytokine; Tumor immunity; Infection immunity; ;

spezie

Rattus norvegicus (Rat)

Cross Reaction

This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b).
No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.

detection target

Interleukin 1 Beta

alias

IL1-B; IL1-Beta; IL1F2; IL-1?; Interleukin-1 Family Member 2; Catabolin

sensitivity

The minimum detectable dose of this kit is typically less than 2.33 pg/mL

detection range

6.25-400 pg/mL

certificate

ISO 9001:2008, ISO 13485:2003

sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

pack size

48T, 96T, 5x96T, 10x96T

Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Assay time

3h

storage and Stability

All the kits should stored according to the manual.
The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree upon receipt while the others should be at 4 degree.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Principle of the Elisa

Double-antibody Sandwich
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Beta (IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Beta (IL1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Kit Content

Pre-coated, ready to use 96-well strip plate: 1
Plate sealer for 96 wells: 4
Standard: 2
Standard Diluent: 1x20mL
Detection Reagent A: 1x120µ L
Assay Diluent A: 1x12mL
Detection Reagent B: 1x120µ L
Assay Diluent B: 1x12mL
TMB Substrate: 1x9mL
Stop Solution: 1x6mL
Wash Buffer (30 x concentrate): 1x20mL
Instruction manual: 1

Assay procrdure summery

1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.

Uniport ID

Q63264

Reference

Prophylactic lithium alleviates postoperative cognition impairment by phosphorylating hippocampal glycogen synthase kinase-3? (Ser9) in aged rats; Improvement by Phytotherapeutic Agent of Detrusor Overactivity, Down-Regulation of Pharmacological Receptors and Urinary Cytokines in Rats with Cyclophosphamide Induced Cystitis; Recombinant Adiponectin Ameliorates Liver Ischemia Reperfusion Injury via Activating the AMPK/eNOS Pathway; Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide-Induced Cystitis; Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cells; Ruscogenin attenuates monocrotaline-induced pulmonary hypertension in rats; Protective Effects of Pretreatment with Oleanolic Acid in Rats in the Acute Phase of Hepatic Ischemia-Reperfusion Injury: Role of the PI3K/Akt Pathway; L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION; Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions; MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy; Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1?) attenuates traumatic brain injury in rats; Effects of honokiol on sepsis-induced acute kidney injury in an experimental model of sepsis in rats; Alpha-lipoic acid exerts anti-inflammatory effects on lipopolysaccharide-stimulated rat mesangial cells via inhibition of nuclear factor kappa B (NF-?B) signaling pathway.; Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes; Senescence of tumor cells induced by oxaliplatin increases the efficiency of a lipid A immunotherapy via the recruitment of neutrophils; NF-?B activation and proinflammatory cytokines mediated protective effect of Indigofera caerulea Roxb. on CCl 4 induced liver damage in rats; Ursolic acid improves survival and attenuates lung injury in septic rats induced by cecal ligation and puncture; Melatonin attenuates intestinal ischemia�reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2; Tanshinone ?A attenuates bleomycin-induced pulmonary fibrosis in rats; Effect of TLR4/MyD88 Signaling Pathway on Expression of IL-1? and TNF-? in Synovial Fibroblasts from Temporomandibular Joint Exposed to Lipopolysaccharide; Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis; Curcumin alleviates lung injury in diabetic rats by inhibiting nuclear factor??B pathway; Cardioprotection against ischemia/reperfusion injury by QiShenYiQi Pill; Effects of dexmedetomidine on the protection of hyperoxia-induced lung injury in newborn rats; Basic Fibroblast Growth Factor Ameliorates Endothelial Dysfunction in Radiation-Induced Bladder Injury; Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats; Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-?B signaling pathway; Penehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion Injury; Betulin attenuates lung and liver injuries in sepsis; Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats; A Standardized Extract of; Silymarin attenuates paraquat-induced lung injury via Nrf2-mediated pathway in vivo and in vitro; Mesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-? and PGE2 and is crucial for liver transplant tolerance; Cyanidin-3-O-glucoside attenuates acute lung injury in sepsis rats; Neuroprotective effect of allicin in a rat model of acute spinal cord injury; Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats; Melanocortin MC6 receptor agonists alleviate brain damage in abdominal compartment syndrome in the rat; Effect of TLR4/MyD88 Signaling Pathway on Expression of IL-1 and TNF-in Synovial Fibroblasts from Temporomandibular Joint Exposed to Lipopolysaccharide; Annexin V homodimer protects against ischemia reperfusion�induced acute lung injury in lung transplantation; The Blockade of NF-?B Activation by a Specific Inhibitory Peptide has a Strong Neuroprotective Role in a Sprague-Dawley Rat Kernicterus Model; Ameliorative effects of Gualou Guizhi decoction on inflammation in focal cerebral ischemic-reperfusion injury; ?????; Modulation of the sympathetic nervous system by renal denervation prevents reduction of aortic distensibility in atherosclerosis prone ApoE-deficient rats; Effect of ozone on vascular endothelial growth factor (VEGF) and related inflammatory cytokines in rats with diabetic retinopathy; Molecular Mechanisms Responsible for Neuron-Derived Conditioned Medium (NCM)-Mediated Protection of Ischemic Brain; The inhibition of transforming growth factor beta-activated kinase 1 contributed to neuroprotection via inflammatory reaction in pilocarpine-induced rats with epilepsy; Annexin V homodimer protects against ischemia reperfusion–induced acute lung injury in lung transplantation; Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery; Curcumin Attenuates Inflammation, Oxidative Stress, and Ultrastructural Damage Induced by Spinal Cord Ischemia�Reperfusion Injury in Rats; Neuroprotective Effect of Ginsenoside Rd in Spinal Cord Injury Rats; Dexmedetomidine Protects Rat Liver against Ischemia-Reperfusion Injury Partly by the ?2A-Adrenoceptor Subtype and the Mechanism Is Associated with the TLR4/NF-?B Pathway;